Abstract
A GC-MS method has been studied for characterization and quantification of phytosterols, cholesterol and cholesterol oxidation products. Baseline separations have been achieved between cholesterol, cholesterol 5α-6α-epoxide, 5-cholestene-3β-ol-7one (7-keto-cholesterol), cholestene-3β-5α-6β-triol, 5-cholestene-3β-25-diol (25-hydroxycholesterol), 5-cholestene-3β-20α-diol (20α-hydroxycholesterol), 5-cholestene-3β-7β-diol (7β-hydroxycholesterol) and 5-cholestene-3β-19-diol (19-hydroxycholesterol) as well as between α-cholestane, cholesterol, stigmasterol, campesterol and β-sitosterol. Excellent linearity of response has been obtained permitting reliable quantification. The characterization of each derivatized sterol has been performed by mass-spectrometry. The results confirm the utility of combined gas chromatography-mass spectrometry in the analysis and characterization of sterols and cholesterol oxidation products.
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