GC - MS analysis of guttation fluids from selected crop plants

Abstract

ABSTRACT Guttation is the mechanism by which liquid exudes from hydathodes located on the tip, along the margins, and on the adaxial and abaxial surfaces of leaves. In contrast to stomata, hydathodes, also known as water stomata or water pores, are often open, representing the path of least resistance to liquid outflow from them. Guttation fluids are made up of several organic compounds. This paper focuses on the defensive properties of the chemical compositions of the guttation extracts of Oryza sativa, Momordica charantia, Luffa acutnugula, and Ricinus communis extracted using five different solvents, which were analyzed using Gas Chromatography–Mass Spectrometry (GC-MS). The mass spectra of the compounds present in the guttation fluids were matched by the National Institute of Standards and Technology (NIST) library. GC-MS analysis of guttation fluid extracts of crop plants revealed the existence of compounds with antifungal and antimicrobial properties which may act as barriers to pathogens. The numerous compounds found in plant extracts, such as fatty acids, organic acids, and terpenoids, have a wide range of protective activities and mechanisms that can serve as the first line of defense against pests and diseases.