Abstract
Standard extraction procedure for examining chromophoric substances of turmeric was investigated. Acetone and methanol were used as extracting solvents with different extraction procedures and pH levels. GC-MS analysis identified curcumene2 (6.7 min), feruloylmethane3 (8.3 min), coumaran4 (6.09 min), vanillin5 (6.2 min), and zingiberene6 (10.5 min) as the major products. Curcumin1 which has been known as the major chromophoric substance of turmeric was not detected in any samples. The maximum amount of curcumene2, which was used as the fingerprint product for turmeric dye, was obtained by utilizing presoaking and decanting step with methanol prior to actual extraction step using a waterbath shaker (WMM). The highest relative abundance of curcumene2 was detected in pH 6 sample followed by pH 5 indicating that the most appropriate pH level was in the range of pH 6–5.
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