GC-MS Analysis of Chemical Composition of in vivo Plant, in vitro and Elicited Roots of Bacopa monnieri (L.) Pennell

Abstract

In vitro plant was developed by tissue culture utilizing nodal explants and adventitious roots were obtained from nodal explants of Bacopa monnieri in Murashige and Skoog (MS) medium supplemented with 0.2 mg/l-1 NAA. In vitro roots of Bacopa monnieri were subjected to chemical elicitation using 69 mg/l-1 of salicylic acid by hairy root culture. In vivo whole plant, in vitro and elicited roots were subjected to solvent extraction using ethanol and their chemical compounds analyzed using gas chromatography and mass spectroscopy (GC-MS). The major composition of in vivo plant were 9,12-octadecadienonic acid (36.96 %), 9,17-octadecadienal (26.65 %,), 9-octadecenoic acid (7.79 %) and in vitro roots yielded 9,12-octadecadienonic acid (25.62 %), 9-octadecenoic acid (23 %), 9,17-octadecadienal (16.08 %). In vitro roots subjected to salicylic elicitation comprised of 1,3-dihydroxyacetone dimmer (15.69 %), 1-hexadecena (7.74 %), 1-tetradecene (6.78 %), eicosane (6.57%), 1-octadecene (5.29 %), 1-decene (4.60 %), E-15-heptadecenal (4.45 %) and heptacosane (3.45 %). In vitro elicited roots showed thirty six compounds and increased higher percentage of sesquiterpenoids and higher alkens.