Abstract
BackgroundApoptosis is important for regulating spermatogenesis. The protein mRHBDD1 (mouse homolog of human RHBDD1)/rRHBDD1 (rat homolog of human RHBDD1) is highly expressed in the testis and is involved in apoptosis of spermatogonia. GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules.ResultsStable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules.ConclusionOur findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.
Highlights
The roles of mRHBDD1 in germ cell apoptosis and mouse spermatogenesis were investigated. We found that it is involved in regulating apoptosis in a mouse spermatagonia cell line, GC-1, and is essential for their capacity to differentiate into spermatids in mouse seminiferous tubules
Apoptotic regulation of mRHBDD1 in a mouse spermatogonia line In order to investigate this putative role of mRHBDD1 in spermatogenesis, we studied its expression in a mousederived spermatogonia cell line, GC-1 spg (ATCC number: CRL-2053; Figure 2A)
An early wave of germinal cell apoptosis during the first round of spermatogenesis in the immature testis is probably a critical requirement during testis development because it maintains a balanced ratio of germ cells to Sertoli cells [20]
Summary
Spermatogenesis generates functional sperm cells from initially undifferentiated germ cells This involves the proliferation of spermatogonia, meiosis of spermatocytes and the differentiation of spermatids into spermatozoa. It is a complex developmental program in which myriad events take place to ensure that the germ cells reach their proper stages of development at the appropriate times. The roles of mRHBDD1 in germ cell apoptosis and mouse spermatogenesis were investigated. We found that it is involved in regulating apoptosis in a mouse spermatagonia cell line, GC-1, and is essential for their capacity to differentiate into spermatids in mouse seminiferous tubules
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