Abstract
Homocysteine is an amino acid related to metabolism in human vivo, which is closely related to cardiovascular disease, senile dementia, bone fracture, et al. Currently, the usual medical test methods for homocysteine include high performance liquid chromatography (HPLC), fluorescence polarization immunoassay (FPIA) and enzyme-linked immunosorbent assay (ELISA), which are time-consuming or expensive. In this paper, we first analyze the vibration and rotation of homocysteine molecules by using density functional theory, and then we ensure that the theoretical absorption peaks are located in the range of the terahertz spectrum. Then, based on the terahertz time-domain spectroscopy system, we measured the absorption spectrum of homocysteine under different concentrations. It is found that as the detection of the concentration, the terahertz results present higher accuracy than that of the laser Raman spectrum, which can be used as the reference for the evaluation of pathological stage. These results are of great significance for the exact and quick diagnosis of homocysteine-related diseases in clinical medicine.
Highlights
Homocysteine (C4H9NO2S [1]) is a sulphydryl-containing amino acid derived from the metabolic demethylation of dietary methionine, which is abundant in animal protein [2]
Experiment setup and sample preparation After the theoretical calculation proves that the fingerprint spectrum of homocysteine is located in the detectable THz range, we used a standard THz time-domain spectroscopy setup (THz-TDS) to measure it
In order to verify this possible problem, we tested this process in experiments: pure homocysteine powder was dissolved in water and made into homocysteine solution
Summary
Homocysteine (C4H9NO2S [1]) is a sulphydryl-containing amino acid derived from the metabolic demethylation of dietary methionine, which is abundant in animal protein [2]. The absorption spectrum of homocysteine was firstly calculated and analyzed by density functional theory, we used the terahertz time-domain spectroscopy (THz-TDS) to detect the absorption spectrum of homocysteine as the change of concentration.
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