Abstract
GATA6 antisense RNA 1 (GATA6-AS1) has been reported to be involved in the progression of several types of cancer. In the present study, the role of GATA6-AS1 in ovarian cancer (OC) was explored. Reverse transcription quantitative PCR was used to detect the expression of GATA6-AS1, microRNA (miR)-19a-5p and tet methylcytosine dioxygenase 2 (TET2) in OC and adjacent normal tissues. Furthermore, OC cells with GATA-AS1 either knocked down or overexpressed were established. The Cell Counting Kit-8 assay was used to evaluate cell proliferation and a Transwell assay was used to assess the migratory and invasive abilities of OC cells. A dual luciferase reporter gene assay was used to determine whether GATA6-AS1 and miR-19a-5p, and miR-19a-5p and TET2, may interact with each other. The results demonstrated that GATA6-AS1 expression level was decreased in OC tissues and cells compared with control groups. In addition, GATA6-AS1 overexpression significantly inhibited the proliferation and migratory and invasive abilities of OC cells, whereas GATA6-AS1 downregulation had the opposite effects. Furthermore, GATA6-AS1 adsorbed miR-19a-5p to repress its expression and GTA6-AS1 indirectly upregulated TET2 expression. Taken together, the findings from this study suggested that GATA6-AS1 could inhibit the proliferation and migratory and invasive abilities of OC cells via regulation of the miR-19a-5p/TET2 axis.
Highlights
Ovarian cancer (OC) is a common tumor of the female repro‐ ductive system, and ~80% of patients are diagnosed in the first instance with advanced stage [1]
The Long non‐coding RNAs (lncRNAs) GATA6 antisense RNA1 (GATA6‐AS1), whose gene is located on chromosome 18q11.2, functions as a tumor suppressor in several types of cancer, such as gastric cancer [7]
The results demonstrated that GATA6‐AS1 was significantly downregulated in OC tissues compared with adjacent normal tissues (Fig. 1C)
Summary
Ovarian cancer (OC) is a common tumor of the female repro‐ ductive system, and ~80% of patients are diagnosed in the first instance with advanced stage (stages III‐IV) [1]. LncRNAs are crucially involved in the progression of various types of cancer [4,5,6]. Small nucleolar RNA host gene 1 is highly expressed in non‐small cell lung cancer (NSCLC) tissues and cells [4], and lncRNA activated by transforming growth factor‐beta inhibits the proliferation and induces the cell cycle arrest of gastric cancer cells [5]. The lncRNA GATA6 antisense RNA1 (GATA6‐AS1), whose gene is located on chromosome 18q11.2, functions as a tumor suppressor in several types of cancer, such as gastric cancer [7]. To the best of our knowledge, the role and regulatory mechanism of GATA6‐AS1 in OC remain unknown
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
