Abstract
BackgroundEpigenetic silencing of tumor suppressor genes plays important role in gliomagenesis. Recently, GATA4 and DcR1 were suggested to be a tumor suppressor genes involved in tumorigenesis in various types of human cancers. However, up to now the methylation frequency of GATA4 and DcR1 genes has not been determined in glioblastoma. In this study, we investigated methylation of GATA4 and DcR1 promoters and their association with patient prognosis in glioblastoma.MethodsMethylation status of GATA4 and DcR1 promoters was investigated by methylation specific PCR in 99 glioblastoma patients. Statistical analyses were conducted to investigate the association between clinical variables and overall survival time.ResultsGATA4 and DcR1 were aberrantly methylated in 23.2% and 27.6% of glioblastoma tumors, but not in normal brain. GATA4 promoter hypermethylation showed significant association with patients age (p = 0.027). Relationship between genes promoter methylation and glioblastoma patient survival was not determined.ConclusionsThe present work demonstrated that GATA4 and DcR1 promoter hypermethylation is tumor specific event in glioblastoma but they promoter methylation cannot be considered as a prognostic marker of glioblastoma survival.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1381170351801852
Highlights
Epigenetic silencing of tumor suppressor genes plays important role in gliomagenesis
We evaluated the methylation status of GATA4 and DcR1 promoters in glioblastoma tumor tissue to support the hypothesis that they are inactivated in glioblastomas by promoter hypermethylation and plays a role as tumor suppressor genes in gliomagenesis
GATA4 and DcR1 promoters are methylated in glioblastoma The methylation status of the GATA4 and DcR1 promoter in glioblastoma samples was detected by methylationspecific PCR assay
Summary
Epigenetic silencing of tumor suppressor genes plays important role in gliomagenesis. GATA4 and DcR1 were suggested to be a tumor suppressor genes involved in tumorigenesis in various types of human cancers. Up to now the methylation frequency of GATA4 and DcR1 genes has not been determined in glioblastoma. We investigated methylation of GATA4 and DcR1 promoters and their association with patient prognosis in glioblastoma. Glioblastoma multiforme (GBM) is the most common and most aggressive malignant primary brain tumor in humans. Looking for new markers which would help better diagnose and predict the course of glioblastoma is important. Much is known about the pathways and mechanisms of apoptosis, but the hypothesis that promoter methylation of GATA4 gene could be survival prognostic marker
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
