Gastroprotective effect of Ferulopsis hystrix (Bunge) Pimenov in ethanol-induced gastropathy

Abstract

The aim of the study was to evaluate the gastroprotective effect of dry extracts from the roots and rhizomes of Ferulopsis hystrix in ethanol-induced gastropathy. Materials and methods . The studies were carried out on 68 white Wistar rats. Ethanol-induced gastropathy was simulated by a single intragastric administration of ethanol in the dose of 10 ml/kg. Animals of the experimental groups received medicinal forms from the roots and rhizomes of F. hystrix : I – decoction in a volume of 10 ml/kg; II–V – dry extracts in the dose of 200 mg/kg, obtained by extraction with purified water, 30, 40 and 70% ethanol, respectively; VI – dry extract, prepared by double extraction with 40% and single extraction with 30% ethanol for 7 days before the modeling of gastropathy. Number of structural changes was determined in the gastric mucosa. They were differentiated into small, large, and strip-like erosions. The Pauls’ index was calculated for structural changes. Pathomorphological studies of the stomach were carried out. Results. The total number of structural changes in the stomach of animals in experimental groups I, II, IV and V is 44% lower on average, in experimental group III it is 67% lower and in experimental group VI it is 3.6 times lower than in the control. The Pauls’ index for large erosions in experimental groups I–V is 38–75% lower, in experimental group VI it is 83% lower than the index in the control animals. No strip-like erosions are detected in animals of experimental groups III–VI. Pauls’ index for these destructions in experimental groups I and II is 7.0 and 6.5 times lower than the index in the control animals. Microscopic morphological examination registered the increase of shallow-like erosions in the stomach of animals of experimental groups. Shallow-like erosions do not reach the muscularis mucosae . Microcirculation disorders and leukocyte infiltration are less pronounced than in the control group. Conclusion. F. hystrix has the gastroprotective effect, increasing the resistance of the gastric mucosa to the effect of ethanol. The F. hystrix extract prepared with 30% and 40% ethanol shows the most pronounced pharmacotherapeutic effect in ethanol-induced gastropathy.