Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity.

Heyam Mohamed Ali Sidahmed,Kamal Aziz Ketuly,Mun Fai Loke,Mahmood Ameen Abdulla,Ainnul Hamidah Syahadah Azizan,Jamuna Vadivelu,Najihah Mohd Hashim,A Hamid A Hadi,Siddig Ibrahim Abdelwahab,Manal Mohamed Elhassan Taha,Syam Mohan

doi:10.1186/1472-6882-13-183

Heyam Mohamed Ali Sidahmed, Kamal Aziz Ketuly + Show 9 more

Open Access

https://doi.org/10.1186/1472-6882-13-183

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Abstract

BackgroundMitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant is rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids and has been reported to possess anti-inflammatory activity. The purpose of this study is to assess the gastroprotective effects of desmosdumotin C (DES), a new isolated bioactive compound from M. kentii, on gastric ulcer models in rats.MethodsDES was isolated from the bark of M. kentii. Experimental rats were orally pretreated with 5, 10 and 20 mg/kg of the isolated compound and were subsequently subjected to absolute ethanol-induced acute gastric ulcer. Gross evaluation, mucus content, gastric acidity and histological gastric lesions were assessed in vivo. The effects of DES on the anti-oxidant system, non-protein sulfhydryl (NP-SH) content, nitric oxide (NO)level, cyclooxygenase-2 (COX-2) enzyme activity, bcl-2-associated X (Bax) protein expression and Helicabacter pylori (H pylori) were also investigated.ResultsDES pre-treatment at the administered doses significantly attenuated ethanol-induced gastric ulcer; this was observed by decreased gastric ulcer area, reduced or absence of edema and leucocytes infiltration compared to the ulcer control group. It was found that DES maintained glutathione (GSH) level, decreased malondialdehyde (MDA) level, increased NP-SH content and NO level and inhibited COX-2 activity. The compound up regulated heat shock protein-70 (HSP-70) and down regulated Bax protein expression in the ulcerated tissue. DES showed interesting anti-H pylori effects. The efficacy of DES was accomplished safely without any signs of toxicity.ConclusionsThe current study reveals that DES demonstrated gastroprotective effects which could be attributed to its antioxidant effect, activation of HSP-70 protein, intervention with COX-2 inflammatory pathway and potent anti H pylori effect.

Highlights

Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae
The present study shows that pre-treatment with desmosdumotin C (DES) significantly protected the gastric mucosa from ethanol-induced ulceration by restoring the depleted GSH level and reducing the
In the results presented IHC assay showed that DES was able to suppress bcl-2-associated X (Bax) protein expression when compared to the ulcer control group

Summary

Introduction

Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant is rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids and has been reported to possess anti-inflammatory activity. The purpose of this study is to assess the gastroprotective effects of desmosdumotin C (DES), a new isolated bioactive compound from M. kentii, on gastric ulcer models in rats. Gastric ulcer is a common disease affecting many people worldwide [1]. The over-ingestion of non-steroidal anti-inflammatory drugs (NSAIDs) and H pylori infection remains the predominant cause of peptic ulcer disease [3]. A large number of natural antiulcer compounds have been isolated from medicinal plants and the common chemical classes of bioactive compounds that have been identified as possessing antiulcer activity are the alkaloids, saponins, xanthones, triterpenes and tannins, among others [8]

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