Abstract
BackgroundGastric microbiota may be involved in gastric cancer. The relationship between gastrointestinal microbes and the risk of gastric cancer is unclear. This study aimed to explore the gastric and intestinal bacteria associated with gastritis and gastric precancerous lesions. We conducted a case-control study by performing 16S rRNA gene analysis of gastric biopsies, juices, and stool samples from 148 cases with gastritis or gastric precancerous lesions from Anhui and neighboring provinces, China. And we validated our findings in public datasets.ResultsAnalysis of microbial sequences revealed decreased bacterial alpha diversity in gastric bacteria during the progression of gastritis. Helicobacter pylori was the main contributor to the decreased microbial composition and diversity in the gastric mucosa and had little influence on the microbiota of gastric juice and feces. The gastric mucosal genera Gemella, Veillonella, Streptococcus, Actinobacillus, and Hemophilus had the higher degree of centrality across the progression of gastric precancerous lesions. And Acinetobacter may contribute to the occurrence of intraepithelial neoplasia. In addition, the microbial model of H. pylori-positive gastric biopsies and feces showed value in the prediction of gastric precancerous lesions.ConclusionsThis study identified associations between gastric precancerous lesions and gastric microbiota, as well as the changes in intestinal microbiota, and explored their values in the prediction of gastric precancerous lesions.
Highlights
Gastric cancer (GC) is the fifth most common cancer globally
We performed endoscopic diagnosis according to the Kimura-Takemoto classification (Duc et al, 2011) and further pathological diagnosis according to the updated Sydney system (Dixon et al, 1996), operative link on gastritis assessment (OLGA) staging system (Rugge et al, 2007), and the modified operative links of gastric intestinal metaplasia (OLGIM) system (Capelle et al, 2010) (Supplementary Figures 2A, C)
Mucosal biopsies with >1% H. pylori relative abundance (RA) were classified as H. pylori-infected, the consistency between the conventional detection methods and the results based on 16S rRNA gene sequencing was only 78.94% in our dataset, and 49.02% in validation datasets (Supplementary Table 3)
Summary
Gastric cancer (GC) is the fifth most common cancer globally It is a health threat worldwide and its mortality rate in China ranks third among malignant tumors for men and second for women (Du et al, 2020). Reducing the mortality rate of GC in China is an urgent public health issue (Collaborators, 2020). Endoscopic assessment requires experienced endoscopists, and pathological assessment is limited by sampling location when diagnosing precancerous gastric lesions, including atrophic gastritis, intestinal metaplasia (IM), and intraepithelial neoplasia (IN) (Quach and Hiyama, 2019). This study aimed to explore the gastric and intestinal bacteria associated with gastritis and gastric precancerous lesions. We conducted a case-control study by performing 16S rRNA gene analysis of gastric biopsies, juices, and stool samples from 148 cases with gastritis or gastric precancerous lesions from Anhui and neighboring provinces, China.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
