GASTRO PROTECTIVE AND ANTIOXIDANT EFFECT OF EUPHORBIA PROSTRATA AGAINST INDOMETHACIN INDUCED GASTRIC ULCERS IN HEALTHY ADULT MALE ALBINO RABBITS

Abstract

Euphorbia prostrate belongs to family Euphorbiaceae locally known as Doodhi is small annual herb. Current study was conducted t o evaluate the gastro protec tive and antioxidant effectiveness of the aerial parts of E . prostrata in indomethacin induced ulcer in adult male albino rabbits. A total 36 mice were taken and divided into 6 equal groups . At day 0 and 14 of experiment the blood sample was collected from each animal and serum was separated from blood which was used for the determination of Total oxidant status (TOS) , Total antioxidant capacity (TAC) , catalase (CAT) and Malondialdehyde (MDA) . At the end of the experiment, all of the rabbits were slaughtere d . The gastric content s were collected to total acid output , gastric pH and gastric volume . To confirm the data results was subjected to one way analysis of variance (ANOVA). Results when compared with the extensive mucosal damage in the indomethacin trate d group , gross evaluation revealed a marked protection of the gastric mucosa in the experimental groups, with significantly reductio n in gastric pH, gastric volume and acid output. In these groups, CAT and TAC levels were significantly increased (P < 0.05) and while MDA and TOS significantly reduced (P < 0.05) which indicate reduction in gastric damage. In conclusion to these results E. prostrata has potent antioxidant properties at dose rate of 240 mg/kg and it is also very effective for the treatment of g astric ulcer at the same dose.