Gastric parietal cell development: expression of the H+/K+ ATPase subunits coincides with the biogenesis of the secretory membranes.

Abstract

The early development of the parietal cell in the embryonic murine gastric mucosa was investigated with particular attention paid to the biogenesis of the secretory membranes and the localization of the gastric H+/K+ ATPase alpha and beta subunits. Gastric glands were recognized in the day 18 foetus. However, at this stage in development no parietal cells could be distinguished ultrastructurally in the glands, and no immunoreactivity was detected with monoclonal antibodies to either the alpha or beta subunits of the gastric H+/K+ ATPase. In the 19 day embryo, parietal cells were recognizable morphologically by the presence of slender microvilli on the apical (lumenal) surface and differentiating intracellular canaliculi in the apical cytoplasm. Both subunits of the proton pump were found to be specifically associated with the apical and canalicular membranes and with the membranes of relatively large vesicles distributed in the subapical cytoplasm and the cytoplasm surrounding the canaliculi. In the parietal cells of the day 1 neonate, the intracellular canaliculi had extended basally to form the extensive compartments typical of parietal cells in the adult animal. Again, profiles of vesicles showing H+/K+ ATPase immunoreactivity were present in the pericanalicular cytoplasm. These results indicate that the intracellular canaliculi are formed by expansion of the apical surface and suggest that the delivery of newly synthesized gastric H+/K+ ATPase alpha and beta subunits to the apical plasma membrane is mediated by typical Golgi transport vesicles. The large immunoreactive vesicles that occur in the apical and pericanalicular cytoplasm of the developing cells may represent artefacts generated by fixation-induced fragmentation of the differentiating canalicular membrane system during preparation.