Abstract
Abstract A gas chromatographic procedure has been developed for the determination of pyridoxal, pyridoxol, and pyridoxamine. These compounds are first converted to their acetyl derivatives and then injected into the gas chromatograph. The acetyl derivatives are formed by the interaction of the vitamin B6 analogs with acetic anhydride in the presence of a catalyst, extracted with a suitable organic solvent, and separated by gas chromatographic techniques. Results were confirmed by infrared, ultraviolet, nuclear magnetic resonance, and mass spectrometry. This technique has been successfully used to quantitatively measure the pyridoxol content of pharmaceutical products
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