Abstract
Gas-liquid chromatography of free protein amino acids has a high and verified reproducibility of quantitative and qualitative estimation: Cytoplasmic matrix obtained from the 105,000 g supernatant fraction of rat liver and kidney cells was investigated. The ultracentrifuged product was passed through ion-exchange resin, thus isolating free amino acids from the eluate. After lyophilization the product was derivatized to N-trifluoroacetyl n-butyl esters and separated on the OV 17 + 210 and ethylene glycol adipate phases. Flame ionization detection was used. Comparison of liver and kidney tissues showed significant differences in the concentrations of the amino acids studied.
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