Abstract
The analysis of photosynthetic processes under field conditions, in particular under varying climatic conditions has become an important issue for plant scientists and agronomists. An easy and robust measuring technique is needed to assess the underlying biophysical and biochemical processes of photosynthesis. Advances in analysis of leaf traits, such as photosynthetic activity and limitations, have been made due to improvements of leaf gas exchange analysis and chlorophyll fluorimetry. In this chapter the basics of photo-biochemistry and physics, as well as the fundamental model of photosynthesis by Farquhar et al. are described. Recent methods on how to determine various photosynthetic parameters are discussed, including a section on potential errors and mistakes. Finally, the potential of combined measurements of leaf gas exchange and chlorophyll fluorescence is introduced, emphasizing the importance of limitations of CO2 diffusion across a leaf (“mesophyll conductance”).
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