Abstract
In vitro techniques have been developed to study the fermentation kinetics of a wide range of animal feedstuffs but relatively few studies have been conducted specifically with purified feed constituents. This study uses the pressure transducer technique of Theodorou et al (1994) to record cumulative gas production (GP) when six purified starch sources were incubated in vitro.Three replicates of six commercially available purified (98%) starch sources were incubated in vitro with an inoculum prepared from freshly voided faeces collected from six ponies fed hay ad libitum. The starches were a purified wheat starch (ABRA), four purified wheat starches that had been chemically modified with sodium tri-metaphosphate (V1, V21, V33 & V65) and a purified pea starch (PEA). GP was measured using the pressure transducer technique throughout a 72 h incubation period. At the end of the incubation period DM loss (DML) in vitro was determined by filtration.
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