Abstract
Some of the difficulties associated with gas—liquid chromatography of C1 to C5 fatty acids isolated from biological materials are discussed. Attention is directed particularly to the problems which arise when thermal conductivity detectors are used.Column packings described previously for the separation of volatile fatty acids were unsatisfactory for analysing dilute ethereal solutions of the acids, especially if they contained small quantities of water.A new stationary phase which adequately separated the acids from water and up to 0.5 ml of ether is described.A procedure is described for the estimation of C1 to C5 fatty acids, isolated from biological material, using a gas chromatograph with a thermal conductivity detector.
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