Abstract
A method for the rapid determination of the number of viable coliforms in milk or other food materials is described. The method is based on the gas-liquid chromatographic (GLC) determination of metabolic CO 2 in equilibrium with the head space of sealed vials, inoculated with aliquots of the sample under examination. The determination of the CO 2 concentration allows the calculation of initial cell level on the basis of a calibration curve. The calibration curve was prepared by plotting the logarithm of the initial colony forming units versus the minimal detection time (MDT) for CO 2 production, determined by GLC. The examination of 24 samples of raw milk showed that this procedure can provide the same information as conventional plate count methods. In addition, the GLC method includes a saving in time and costs.
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