Abstract
Abstract Trans isomers in several methyl ester mixtures and in some commercial margarines were separated on a 15′ × ⅛″ stainless steel column containing 10% Silar 10C as stationary phase. With the methyl ester mixtures, good agreement was obtained among known trans contents, trans contents determined by infrared (IR) spectroscopy, and trans contents determined by gas-liquid chromatography (GLC). With commercial margarines, agreement between IR and GLC results was less satisfactory; the latter were approximately 80–85% of the former when trans contents were >20%. When trans contents were <20%, discrepancies were larger. This was considered due to overlap of certain cis and trans positional isomers.
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