Abstract
Abstract A method was developed for gas-liquid chromatographic determination of sucrose fatty acid esters as TMS derivative of sucrose and methyl esters of fatty acids. Sucrose fatty acid esters were completely degraded to sucrose and fatty acids in alkaline ethanol overnight at 25°C. Sucrose was derivatized with pyridine, trimethylchlorosilane, and N-trimethylsilylimidazole and the sucrose TMS derivative was determined on a 2% OV-17 column. Fatty acids were extracted with ethyl ether, methylated with BF3-methanol complex at 65°C, and determined on a 2% DEGS + 0.5% H3PO4 column. This method was applied to selected sucrose fatty acid esters. For example, sucrose and fatty acids derived from 50 mg sample F20 were 10.6-11.0 and 38.1-39.0 mg, respectively. Total amounts were 48.7-50.0 mg with a standard deviation of 0.4 (n = 6).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.