Abstract
ABSTRACTA simple, rapid chromatographic procedure for the determination of carbon disulfide in shiifake mushrooms was developed. The method involves homogenization of dried mushroom, followed by distillation of the homogenate in the presence of acetone. Carbon disulfide in acetone is determined by GLC with a flame photometric detecting system. The carbon disulfide content in dried mushroom was affected considerably by the pH of homogenation and distillation; 0.02–3.4 ppm of carbon disulfide in dried mushrooms under acidic condition increased to 0.98–72.5 ppm under neutral conditions. The increased carbon disulfide under neutral conditions is purported to be derived from a mushroom component. Therefore, residual carbon disulfide in dried shiitake mushrooms must be determined under acidic conditions of homogenation and distillation. The lower delection limit was 0.025 ppm.
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