Abstract
Formation of gas hydrate (aqueous clathrate or clathrate hydrate) crystals in intact tissue was studied using water-soluble ethylene oxide as a hydrate former. The presence of internal hydrate crystals was assessed by the characteristics of time-temperature plots obtained during warming of treated samples, by microscopic examination of treated samples, and by visual observation of hydrate formation in a device containing treated tissue embedded in three layers of polyacrylamide gel. On the basis of these tests, it was concluded that ethylene oxide hydrate can be formed in either plant or animal tissue if a sufficient quantity of ethylene oxide is allowed to diffuse into the tissue prior to initiation of crystallization.
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