Abstract
The Ames test has been used to evaluate the mutagenic effect of exhaust gas using diesel and biodiesel fuels (BDFs) from power generator. The Salmonella typhimurium (TA98 and TA100 tester strain) were cultured because of the most sensitive for organic pollutant. The direct sampling from gas emission for Ames test was the first time proposed. Six low molecular-weight methyl esters (LMW-MEs) found in exhaust gas when using biofuels have been tested to reveal that they are not mutagenic but toxic. The quality control standards such as dimethyl sulfoxide (DMSO) and sodium azide (NaN3) were used as negative and positive control in all culture processes. DMSO is the best adsorbent to trap the pollutants in exhaust gas. The mutagenic effects of Diesel fuel (DF) with Jatropha BDF (JCO) and waste cooking oil BDF (WCO) have been evaluated. The highest mutagenicity of WCO was observed in both TA 98 and TA100 testers strain. For the same engine, the mutagenic test result is different between two kinds of BDFs. BDFs showed increased mutagenicity higher than DF with WCO>JCO>DF. The number of revertant colonies are 623>508>424 for TA100 and 66>50>41 for TA98, respectively.
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