Gas chromatography\u2013mass spectrometry (GC\u2013MS) analysis of ethyl acetate root bark extract of Strychnos innocua (Delile)

Hamisu Ibrahim,Ogunkemi Risikat Agbeke Iyun,Muhammad Sani Sallau,Ahmed Jibrin Uttu

doi:10.1186/s43088-021-00156-1

Hamisu Ibrahim, Ogunkemi Risikat Agbeke Iyun + Show 2 more

Open Access

https://doi.org/10.1186/s43088-021-00156-1

Copy DOI

Abstract

BackgroundMajority of phytochemicals have been known to bear valuable therapeutic activities such as insecticidal, antibacterial, antifungal, anticonstipative, spasmolytic, antiplasmodial and antioxidant activities. Strychnos innocua is straight-stemmed tree belonging to the family Loganiaceae and can grow up to 18 m tall. The plant is used for various pharmacological purposes. The aim of this study was to determine the chemical composition of the ethyl acetate extract of root bark of S. innocua using GC–MS analysis. The root bark was collected, air-dried and then crushed to powder. Standard extraction method (maceration) was used to obtain the ethyl acetate extract. The GC–MS was carried out on the extract using GC 7890B, MSD 5977A, Agilent Tech.ResultsThirty-seven compounds were identified among which dibutyl benzene-1,2-dicarboxylate showed the highest peak area (31.03%) and monomethyl pimelate showed the lowest peak area (0.39%). The major compounds identified were cyclooctane (methoxymethoxy), 2,4-dimethylheptanedioic acid dimethyl ester, azelaic acid, 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester, dibutyl benzene-1,2-dicarboxylate, butyl 8-methylnonyl benzene-1,2-dicarboxylate, 9,15-octadecadienoic acid, methyl ester, cis-vaccenic acid, linoleic acid ethyl ester and ethyl oleate.ConclusionsIn conclusion, these phytoconstituents might be responsible for the medicinal efficacy of the root bark of S. innocua and can be used as a source therapeutic drug.

Highlights

Majority of phytochemicals have been known to bear valuable therapeutic activities such as insecticidal, antibacterial, antifungal, anticonstipative, spasmolytic, antiplasmodial and antioxidant activities
Strychnos innocua belongs to the family Loganiaceae and is often straight-stemmed tree growing up to 18 m tall
The Gas chromatography–mass spectrometry (GC–MS) analysis of the ethyl acetate extract of root bark of Strychnos innocua resulted in the detection of 37 compounds that were identified among which dibutyl benzene-1,2-dicarboxylate showed the highest peak area (31.03%) and monomethyl pimelate showed the lowest peak area (0.39%)

Summary

Introduction

Majority of phytochemicals have been known to bear valuable therapeutic activities such as insecticidal, antibacterial, antifungal, anticonstipative, spasmolytic, antiplasmodial and antioxidant activities. The plant is used for various pharmacological purposes. The aim of this study was to determine the chemical composition of the ethyl acetate extract of root bark of S. innocua using GC–MS analysis. The root bark was collected, air-dried and crushed to powder. Antiprotozoal activities of medicinal plants have been reported [24, 26]. The guidelines for the assessment of herbal medicines have once been issued by WHO, and these guidelines explained basic criteria for the evaluation of quality, safety and efficacy of herbal medicines with the goal of assisting national regulatory authorities, scientific organizations and manufacturers in assessing documentation, submissions and dossiers in respect of such products [25].

Objectives

Methods

Results

Discussion

Conclusion