Abstract
A gas chromatographic separation of the trifluoroacetylated n-amyl esters of α-amino-isobutyric acid, alanine, valine, threonine, glycine, isoleucine, leucine, β-amino- n-butyric acid and serine in that order has been achieved isothermally using mixed stationary phases. With a different mixed phase at a higher temperature the following amino acids were separated: cysteine, proline, hydroxyproline, methionine, ethionine, phenylalanine, aspartic acid, tyrosine, ornithine, glutamic acid and lysine. The difficulties with other protein amino acids are discussed.
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