Abstract

Simultaneous separation and the possibility of quantification of the trimethylsilylated (TMS) bases and nucleosides using gas-liquid chromatography are described. The TMS bases and nucleosides were sufficiently volatile for gas chromatography and readily regenerated the original molecules on very mild hydrolysis. The TMS derivatives were isolated from the trimethylsilylation products of bases and nucleosides, and the structures were established by analyses, NMR spectra, and infrared spectra. The structures of the individual components of gas chromatographic peaks are discussed.

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