Abstract
The use of 1,3-dichlorotetrafluoroacetone in combination with reactive anhydrides such as heptafluorobutyric anhydride provides a useful, quick and sensitive method of analysing the twenty protein amino acids by gas chromatography. The chemical treatment proceeds under very mild conditions without the necessity of heat so that both amides, glutamine and asparagine, are preserved and amenable to chromatographic estimation. An ordinary column (2 m) packed with OV-17 stationary phase gave excellent separations of the derivatized compounds in the temperature range of 75–230°C within 20 min. A second short column is required for complete elution of histidine, tryptophan and cystine.
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