Abstract
Glucosinolate degradation products from Brassica spp. may control soil-borne plant pests; however, a comprehensive method for the analysis of the responsible glucosinolate-derived allelochemicals in soils is lacking. Splitless injection and a 5% phenyl-substituted methylpolysiloxane fused silica capillary column in a gas-liquid chromatograph equipped with a flame ionization detector were used to separate various isothiocyanates, nitriles, and oxazolidinethione in less than 22 min. A linear relationship between detector response for isothiocyanates and molecular weight was observed, allowing estimation of isothiocyanate concentrations for which standards are not readily available
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