Abstract
Objective: The objective of this study was to determine heavy metals using inductively coupled plasma mass spectrometry (ICP-MS) and phytochemical constituents of methanol extract of Caesalpinia bonducella seed kernel using gas chromatography - mass spectrometry (GC-MS).
 Methods: In GC-MS investigation, 95% methanol extract of C. bonducella seed kernel was performed on JEOL GC MATE ΙΙ, column HP 5 MS, and Quadruple, double-focusing mass analyzer. Determination of heavy metals from C. bonducella powder was performed using Thermo scientific I CAP Q instrument, for plant powder digestion Anton Paar Microwave model: Multiwave 300 was used. Antimicrobial activity was evaluated using microdilutions broth method.
 Results: GC-MS analysis of methanol extract of C. bonducella seed kernel has shown 11 different phytoconstituents. ICP-MS analysis has shown the concentration of ten heavy metals in C. bonducella seed kernel as follows: Cr 1.5 ppm, Fe 72.72 ppm, Co 0.33 ppm, Ni 0.03 ppm, Cu 10.73 ppm, Zn 18.44 ppm, As 0.03 ppm, Cd 0.02 ppm, and Pb 0.58 ppm.
 Conclusion: Result showed that the plant is safe to use from toxic heavy metals such as As, Cd, and Pb. It is suitable to check frequently the heavy metal content in the plant used in traditional medicines before given to patients.
Highlights
Medicinal plants have been used for the treatment of various diseases from ancient time and have been playing an important role in the discovery of the modern day medicines with novel chemical constituents [1]
Plants need some of the heavy metals for their structural and biological functions such as Zn, Cu, Ni, Mn, Cr, and Fe; these elements are considered as essential trace elements [6] whereas toxic elements which have unknown biological role in the plant such as Cd, As, Hg, and Pb are toxic even at low concentration [7]
Heavy metals are present in medicinal plants in different concentrations, and these levels are influenced by several factors such as type of plant, the type of soil, the climate, and the type of agriculture [8]
Summary
In GC-MS investigation, 95% methanol extract of C. bonducella seed kernel was performed on JEOL GC MATE ΙΙ, column HP 5 MS, and Quadruple, double-focusing mass analyzer.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
