Gas Chromatography-Mass Spectrometric Analysis, Isolation, Characterization and Biological Activity of Ethanolic Extract of Moss Fabronia secunda Mont.

Abstract

The chemical and biological profile of the ethanolic extract of Fabronia secunda examined for the first time. The gas chromatography-mass spectrometric analysis of ethanolic extract of Fabronia secunda identified 200 components, which mainly composed of lup-20(29)-ene-3,28-diol (4.01 %), guanosine (3.99 %), and 1,2-benzene-dicarboxylic acid (3.79 %). Also, the chromatographic analysis yielded three compounds, namely stigmasterol (1), β-sitosterol (2) and lupeol (3). The ethanolic extract of Fabronia secunda exhibited superior inhibition of superoxide and 2,2-diphenyl-1-picrylhydrazyl radicals with an half maximal inhibitory concentration value of 33.5 and 34.5 μg/ml respectively. Additionally, ethanolic extract of Fabronia secunda depicted prominent inhibitory profiles against alpha-glucosidase and pancreatic alpha-amylase with half maximal inhibitory concentration values of 48.5 and 58.0 μg/ml respectively. To conclude, this preliminary chemical analysis provides a piece of evidence to know the metabolism of mosses and the biological investigation proved the therapeutic importance of mosses like Fabronia secunda.