Abstract
Anabolic steroids are used as doping agents in sports. Notwithstanding the total EC ban, they are also used as growth promoters in animal production. The detection of anabolic steroids in the urine of untreated persons due to the consumption of contaminated meat has been described earlier. This paper describes further confirmation of a urine sample which was clostebol positive as a result of the above described interference phenomenon. This was done by gas chromatography—mass spectrometry. Both low resolution mass spectrometry (LRMS) and high resolution mass spectrometry (HRMS) were used after gas chromatographic separation. LRMS was used to determine the relative abundances of the ions and for calculating similarity indices. With HRMS, selectivity was increased by recording exact masses instead of nominal masses.
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