Gas chromatographic resolution of butteroil and synthetic triglycerides beyond their carbon numbers.

Abstract

AbstractPreparative gas chromatography was used to isolate the C26 to C38 triglycerides of butteroil in groups of uniform molecular weight. Determination of fatty acids allowed a preliminary assignment of triglyceride structure and an estimation of the relative proportions of the various glyceride types. A basis for a further gas chromatographic resolution of saturated triglycerides of identical molecular weight but differing in composition and positional distribution of fatty acids was recognized in the easy separation of various derivatives of 1‐ and 2‐monoglycerides. The study indicated that it might be practical to separate positional isomers of those triglycerides which contain fatty acids of both short and long chain lengths. Other promising routes to increased glyceride resolution were suggested by experiments using chromatographic conditions approaching gas‐solid systems.