Abstract
Our gas chromatogrophic method established previusly has been modified to assay fluoride ion which is released from fluorine-containing compounds and drugs in human plasma. A strongly acidic medium was not necessarily required, though fluoride is usually derivatized to trimethylfluorosilane (TMFS) under such acidic conditions. Trimethylsiylation proceeded in pH 2.5 sulfosalicylate (SSA) buffer. Even labile fluorine-containing compounds were stable in SSA buffer, while they were degraded in a strong acid to form fluofide. SSA was effecitive for buffering HCl which was produced by the reaction of excess reagent, trimthylchlorosilane (TMCS), with H2O. At the same time, SSA in plasma sample served as not only a deproteinizing agent but also a buffer agent for biological materials. By means of the present method, released fluoride in human plasma is assayable even in the presence of labile fluorine-containing compounds. More than 5 ng/ml of fluoride can be quantitated. α-Fluoro-β-alanine, 4-fluoroglutamic acid, 3-difluoroalanine and 5-(trifluoromethyl)uracil released fluoride in human plasma at 37°C in vitro. The amount of fluoride increased with time. On the other hand, no fluoride was liberated from 5-fluorouracil of from compounds of drugs studied other than the above ones.
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