Abstract
Abstract A technique is described for the determination of 2′- O -methylation in RNA using gas chromatography to quantitate the methanol released by perchloric acid cleavage of the 2′-methoxy group. The technique can be used with small samples of RNA and does not require radioactive labeling. It is shown that base methylated compounds do not interfere with the assay and that the method is accurate over widely ranging contents of 2′- O -methylnucleotides.
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