Abstract
An analytical procedure based on gas chromatography of silyl derivatives is described for separation and quantitation of the mycotoxins kojic acid, terreic acid, and terrein. The method can be used for as little as 0.1 μg of the compounds and appears to be suitable for separating these substances from each other and from other mold metabolites in crude extracts. A cleanup procedure, based on silica gel column chromatography, is also described for separation of kojic acid from other metabolites of Aspergillus flavus and for purification and crystallization of kojic acid.
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