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Abstract
The present report describes the identification of aldehydic products of lipid peroxidation by two chromatographic methods coupled to mass spectrometry, GC/EI-MS and LC/ESI-MS. The easy detection of molecular ions in LC/ESI-MS and fragmentation pattern in GC/EI-MS illustrate the complementarity of these two techniques. Several carbonyl compounds, other than the usual reported breakdown products of lipid peroxidation, e.g. malondialdehyde and 4-hydroxynonenal, were identified as 2,4-dinitrophenylnonenal, derivatives in the Fe 2+ /ascorbate-catalyzed peroxidation of linoleic and linolenic acids.
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