Garcinia mangostana peel extracts exhibit hepatoprotective activity against thioacetamide-induced liver cirrhosis in rats

Abstract

• Hepatoprotective effect of Garcinia mangostana is reported using experimental rats. • TAA induced liver cirrhosis assessed immuno/histochemically and biochemically. • Garcinia mangostana significantly down-regulate the PCNA, α-SMA and TGF-β1. • The activities of SOD and CAT considerably enhanced with decline in MDA level. • The serum level of ALP, ALT, AST, bilirubin and albumin was normal with p < 0.0001. The study in vivo evaluated the influence of ethanolic extract of G. mangostana peel on the histology, immunohistochemistry, and biochemistry of thioacetamide (TAA) induced liver cirrhosis in Sprague Drawly (SD) rats. The rats were distinctly administered with two doses of G. mangostana (250 mg/kg and 500 mg/kg) with TAA showed a significant reduction in liver index and hepatocyte proliferation with much lesser cell damage. These groups were significantly down-regulated the PCNA, α-SMA, and TGF-β1. The liver homogenate exhibited increased antioxidant enzymes (SOD and CAT) activities accompanied with decline in malondialdehyde (MDA) level. The serum level of bilirubin, total protein, albumin and liver enzymes (ALP, ALT, and AST) were restored to normal and were comparable to that normal control and silymarin with TAA treated groups. Thus normal microanatomy of hepatocytes, suppression of PCNA, α-SMA, TGF-β, improved antioxidant enzymes, reduced MDA with restoration of liver biomarkers demonstrates hepatoprotective influence of G. mangostana .