Abstract
The aim of this study is to investigate the effects of ganoderic acid A (GAA) on OVA-induced asthma in mice. Mouse asthma model was established by ovalbumin (OVA) in vitro. Diff-Quik staining was used to observe the total numbers of cells and the number of classification cells in each group, and HE staining was used to observe lung inflammation in lung tissue sections. ELISA was used to detect the effect of GAA on the levels of interleukin-4 (IL-4), IL-5, and IL-13 in serum and lung tissue. The expression levels of TLR/NF-κB were detected by Western blot. Immunohistochemistry was used to observe the expression changes of TLR4 and P-P65. Compared with the normal group, the inflammatory cell count, IL-4, IL-5, and IL-13 expression in the model group increased, and TLR/NF-kB signal protein expression increased. Compared with the model group, in GAA group, the number of inflammatory cells, the expression of IL-4, IL-5, and IL-13 decreased, and the expression of TLR/NF-kB signaling protein decreased. GAA regulated lung inflammation in asthmatic mice by inhibiting TLR/NF-kB signaling pathway.
Highlights
Bronchial asthma is a chronic disease characterized by pathological changes such as abnormal inflammatory reaction of the airway, abnormal changes in airway wall structure, and increased tracheal-bronchial reactivity [1]
Allergens in the environment, such as indoor dust storm (HDM), endotoxin, and virus infection, which can be combined with pathogen-related pattern recognition receptors of the bodies, and different gene expression patterns are induced by activating different signal transduction pathways, causing the release of various proinflammatory factors [5]
House dust female (HDM) is one of the major ligands of TLR4, which is ubiquitous in the air and induce acute attack of asthma
Summary
Bronchial asthma is a chronic disease characterized by pathological changes such as abnormal inflammatory reaction of the airway, abnormal changes in airway wall structure, and increased tracheal-bronchial reactivity [1]. Allergens in the environment, such as indoor dust storm (HDM), endotoxin, and virus infection, which can be combined with pathogen-related pattern recognition receptors of the bodies, and different gene expression patterns are induced by activating different signal transduction pathways, causing the release of various proinflammatory factors [5]. After TLR4 recognizes with HDM in the environment, it can activate intracellular transcription factor NFkB and related protein kinases; release related cytokines, such as TNF-a, IL-8, and IL-6; and promote the metamorphosis of the body [8]. Ganoderma lucidum (scientific name, Ganoderma lucidum Karst), known as Linzhongling and Qiongzhen, is the fruiting body of Ganoderma lucidum, a fungus of the Polyporaceae [10] It has effects in invigorating qi, tranquilizing mind, relieving cough and asthma, and prolonging life. The purpose of this paper is to explore the effect of GAA on OVA-induced asthma in mice and its mechanism
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