Abstract
Objective To explore correlation between CT radiation dose and the number of gamma-H2AX (phosphorylated form of H2AX histone variant) foci in human peripheral blood mononuclear cells (PBMC) by using γ-H2AX-based immunofluorescent assay. Methods 64 volunteers received phlebotomy for peripheral blood samples from cubital vein. Based on CT dose, a total of 16 groups of blood samples were used for in -vitro CT radiation (n=4 samples each). We measured the expression of PBMC γ-H2AX. Fluorescence microscopy was used for counting γ-H2AX foci. Results After in-vitro CT radiation, there was a significant increase in human PBMC γ-H2AX foci which was in positive linear correlation with the CT dose-length product (DLP) (R2=0.9357, P<0.05). Conclusion γ-H2AX quantitatively reflects CT radiation-induced peripheral blood DNA damage, which is justified for widespread use as a biomarker for low-dose ionizing radiation. Key words: Tomography, X-ray computed; γ-H2AX; DNA damage
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