Abstract

A BHC decomposer isolated from a paddy field soil of Shiga prefecture was identified to be Clostridium rectum. The most dominant degradation intermediate from γ-BHC was identified to be γ-3,4,5,6-tetrachlorocyclohexene (γ-TCCH) by gas chromatography-mass spectrometry. Degradation of T-BHC took place rapidly in the early growth phase of C. rectum, but was remarkably reduced in the resting state, e.g., spore-formed cell or washed cell suspension. The growth and the BHC decomposing ability of C. rectum were inhibited simultaneously by oxygen, chloramphenicol, streptomycin and pentachlorophenol. BHC degradation is discussed in relation to the co-metabolism,

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