Abstract
Gametophytes of Ophioglossum engelmannii Prantl grow in axenic culture on a nutrient medium containing inorganic nutrients and sucrose. The dark-grown prothalli are long, white, cylindrical structures without rhizoids. The gametophyte has a meristem with a single apical cell. Antheridia, which are partially sunken, and archegonia, which have short necks, are interspersed along the gametophyte. The prothalli are endophyte free. Since the gametophytes of O. engelmannii are undescribed from nature, no comparisons can be made between gametophytes from culture and those from nature. However, gametophytes grown in axenic culture have a morphology which is normal for the genus.
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