Abstract
The research aims to study the use of synbiotics as feed additives in rations on describe lesions histopathology and population of lactic acid bacteria in duodenum infected with Escherichia coli. The study used 96 broiler Jumbo series A Cibadak strains which were maintained until the age of six weeks. The study was conducted by experimental method, using a completely randomized design (CRD) with eight treatments of rations and four replications. The broilers were devided into eight treatment diets: R1 (basal diet/control), R2 (basal diet + prebiotic 0.4%); R3 (basal diet + probiotic 108 CFU); R4 (basal diet + prebiotic 0.4% + probiotic 108 CFU); R5 (basal diet + infected with E. coli 104 CFU); R6 (basal diet + prebiotic 0.4% + infected with E. coli 104 CFU); R7 (basal diet + probiotic 108 CFU + infected with E. coli 104 CFU); and R8 (basal diet + prebiotic 0.4% + probiotic 108 CFU + infected with E. coli 104 CFU). The observed variables were histopathology based on lesio microscopic and lactic acid bacteria population in duodenum age 2, 4 and 6 weeks. Data was analyzed by using one-way analysis of variance then continued with Duncan test. The results showed that the use of probiotics, prebiotics and synbiotics significantly (P<0.05) decreased histopathological lesio score and increased the population of lactic acid bacteria in duodenum age 2, 4 and 6 weeks. In conclusion the use of synbiotics as feed additives in the ration a positive on describe lesions histopathological and increase in the population of lactic acid bacteria in duodenum age 2, 4 and 6 weeks infected and not infected with E. coli.
Highlights
The research aims to study the use of synbiotics as feed additives in rations on describe lesions histopathology and population of lactic acid bacteria in duodenum infected with Escherichia coli
The study was conducted by experimental method, using a completely randomized design (CRD) with eight treatments of rations and four replications
The broilers were devided into eight treatment diets: R1, R2; R3; R4; R5; R6; R7; and R8
Summary
Materi penelitian yang digunakan adalah ayam pedaging umur sehari Infeksi bakteri E.coli dilakukan pada umur tujuh hari dengan cara injeksi kedalam mulut ayam pedaging sebanyak 104 CFU/ekor. Semua ransum perlakuan menggunakan bahan pakan yang sama, hanya berbeda pada penggunaan probiotik, prebiotik dan sinbiotik. Variabel Penelitian Variabel yang diamati adalah gambaran histopatologi dan populasi bakteri asam laktat (BAL) pada duodenum ayam pedaging umur 2, 4, dan 6 minggu. Pengamatan terhadap mikrobiota usus bakteri asam laktat (BAL) dilakukan pada usus bagian duodenum ayam pedaging umur 2, 4, dan 6 minggu. Sampel berupa isi duodenum diambil sebanyak 0,5 g, selanjutnya dilakukan pengenceran secara serial dan dilakukan kultur pada cawan petri dengan menggunakan media De Man, Rogosa dan Sharpe agar (MRS agar) kemudian diinkubasi pada suhu 37oC selama 24 jam. Selanjutnya data dianalisis dengan membandingkan gambaran perubahan histopatologi pada organ duodenum ayam pedaging baik yang diinfeksi maupun yang tidak diinfeksi bakteri E. coli
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