Gambaran Hasil Aktivitas Antioksidan Berberapa Perlakuan Teknik Preparasi Pembuatan Konsentrasi Ekstrak Kulit Buah Nanas (Ananas comosus L.Merr) Menggunakan Senyawa DPPH (1,1-diphenyl-2-picrylhydrazyl)

Abstract

Pineapple skin (Ananas comosus L.Merr) has been known to have a category of strong antioxidant activity against reducing DPPH compounds (1,1-diphenyl-2-picrylhydrazyl). However, references to the preparation process of extract concentration making and the treatment of DPPH compounds to extracts are not much elaborated, so that it can cause errors in inferring the category of antioxidant activity. The purpose of this study was to determine the results of antioxidant activity through the treatment of preparation techniques for the concentration of pineapple peel extract concentration. Pineapple peel extract (Ananas comosus L. Merr) was prepared by maceration method using 70% ethanol solvent. Testing of antioxidant activity was carried out using the UV-Vis spectrophotometric method at a wavelength of 516.4 nm. The extract concentration to be tested was 500 ?g/mL, 250 ?g/mL, 125 ?g/mL, 62.5 ?g/mL and 31.25 ?g/mL and the concentration of DPPH compounds (1,1-diphenyl-2-picrylhydrazyl) was 45 ?g/mL. Treat the extract making concentration divided into 3 namely P1 (multilevel dilution with a volume ratio of 1: 1) P2 (normal dilution with a volume ratio of 1: 1) and P3 (DPPH solution is used to suffice the volume of the extracted test extract). The results of percent inhibitors from each treatment of making concentrations showed P1 <P2, P1 <P3, P2 <P3, and IC50 of each treatment were P1 268.07 ?g/mL, P2 was 231.28 ?g/mL, P3 was 78.403 ?g/mL. Based on the results of IC50 P3 gives the category of antioxidant activity that is 78,403.Keywords: pineapple fruit skin (Ananas comosus L. Merr), antioxidants, dpph (1,1-diphenyl-2- picrylhydrazyl), dilution.