Gallic Acid Exerts Nephroprotective, Anti-Oxidative Stress, and Anti-Inflammatory Effects Against Diclofenac-Induced Renal Injury in Malerats

Abstract

Diclofenac (DIC) is a Nonsteroidal anti-inflammatory drug (NSAID) and consumption of this drug creates side effects such as renal injury. The purpose of this work was to assess the influences of gallic acid (GA) on DIC-induced renal injury in rats. Rats were segregated into five groups. Group 1, control group; Group 2 received DIC-only (50mg/kg bw, i.p.) for 7 consecutive days; Groups 3, received GA-only (100mg/kg bw, po) for 7 consecutive days; group 4 received DIC (50mg/kg bw, i.p.) plus GA (50mg/kg, po) for 7 consecutive days and group 5 received DIC (50mg/kg bw, i.p.) plus GA (100mg/kg, po) for 7 consecutive days. The data indicated that the levels of the serum protein carbonyl, sGOT, sGPT, urea, creatinine, uric acid, nitrite content, MDA, serum IL-1β, and the renal IL-1β gene expression were remarkably increased in DIC-only treated animals compared to control group. In the other hand, treatment with gallic acid led to significant improvements in abnormalities of DIC-induced oxidative stress and serum biochemical parameters. Histological changes were also ameliorated by GA oral administration. The results indicated that oral injection of GA could alleviate the noxious effects of DIC on the antioxidant defense system and renal tissue.