Gallic acid antagonizes the distribution and expression of Vimentin injury induced by cyclophosphamide in mice

Abstract

Objective To observe that GA antagonized testicular cytoskeleton protein vimentin injury induced by CP.Methods The 3-week-old immunocompetent C57BL/6j mice were transplanted with SK-N-SH cells,distribution of randomized block design,10 of each,the control groups:(1) PBS group,0.5ml PBS per bearing tumor mouse,intraperitoneally (i.p) ; (2) CP group,75mg/kg per bearing tumor mouse,i.p,twice a week,continuous two weeks ; (3) NC group,as the reproductivity of mice.Experimental groups:(1)GA group,administered gallic acid 250mg/kg i.p per bearing tumor mouse,every other day,continuous two weeks; (2) GA plus CP group,GA and CP independent administration,the dose and time with the above.To compare the absolute weight and the relative organ index of the testes.The expression of vimentin mRNA and protein in testes were detected by immunohistochemistry,reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting,respectively.Results The testes organ coefficient were significantly decreased after CP administration (P ＜ 0.01),and restored after GA intervene (P ＜0.05).Compared with PBS group,there was no significant difference after GA administration alone (P ＞0.05).The cytoskeletal protein vimentin in testes was abnormal distributed after CP administration,however,the distribution were similarly between GA plus CP group and PBS group.The vimentin mRNA and protein expression in testes was significantly lower after CP administration (P ＜0.01),but that restored after GA intervene (P ＜ 0.01).There were no significant differences between GA plus CP group and PBS group.Conclusion GA may antagonize the toxicity of CP to the testes and stable the normal distribution and expression of cytoskeletal protein vimentin in mice. Key words: Testis sertoli cells ; Gallic acid ; Cyclophosphamide ; Vimentin