Abstract
BackgroundAn impaired leukocytes function is the factor causing the susceptibility of patients with diabetes mellitus to infections. The outmost importance for the understanding of the immunological processes involved in diabetes pathogenesis is to give the characteritics of the immunological profile and changes therein, during the course of desease. Long-used in folk medicine to treat diabetes Galega officinalis L. has been chosen for the correction of the immune system dysfunction.MethodsThe experiments were conducted on male Wistar rats. Fractionation of bone marrow cells suspension was performed in a three-layer ficoll–sodium amidotrizoate density gradient. The lymphocytic-granulocytic cells proliferative activity was studied using enzyme immunoassay with 5-bromo-2′-deoxyuridine (BrdU). For staining of bone marrow preparations May-Gruenwald-Romanowsky-Giemsa (Pappenheim) method was used. To evaluate the content of cationic proteins and myeloperoxidase in neutrophilic leukocytes cytochemical studies were performed. Content of tumor necrosis factor alpha was carried out by immuno-enzymatic analysis. Lymphocytes apoptosis was examined by fluorescent analysis using annexin V.ResultsDiabetes mellitus development was accompanied with violation of neutrophils and lymphocytes proliferation, increased activity of myeloperoxidase and enhanced apoptosis process. Administration of Galega officinalis extract under the condition of diabetes promotes the restoration of neutrophils bone marrow pool and the reduction of lymphoblasts number and causes inhibition of the lymphocytes apoptosis process.ConclusionsInvestigated medicine has a pronounced immunocorrective effect under the conditions of diabetes mellitus and can become the basis for creating a new generation of antidiabetic drugs.
Highlights
An impaired leukocytes function is the factor causing the susceptibility of patients with diabetes mellitus to infections
It has been shown that administration of Galega officinalis extract to animals with diabetes mellitus (DM) caused the decrease by 2.8 times of 5-bromo-2′-deoxyuridine inclusion level in proliferating cells (Fig. 2)
The development of DM was accompanied by changes in leukocytes precursors population in bone marrow and reduction of the number of myeloblasts, which were the cause of the decrease of juvenile and staff neutrophils number
Summary
An impaired leukocytes function is the factor causing the susceptibility of patients with diabetes mellitus to infections. A number of patients with DM have increased in incidence of infections. Some of these infections are more likely to have a complicated course in diabetic than in nondiabetic patients [2]. The quality and the magnitude of an immune response are determined by the communication between cells of immune system [4]. An inflammatory disorder such as diabetes is accompanied with implicated innate lymphocytes functions, leading to tissue dysfunction, barrier breach and severe pathology during local infection. Adaptive immune responses could be modulated by these cells by releasing
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