Galanin is expressed in kisspeptin neurons and co-localizes with kisspeptin within afferents to GnRH neurons in the mouse brain

Abstract

Event Abstract Back to Event Galanin is expressed in kisspeptin neurons and co-localizes with kisspeptin within afferents to GnRH neurons in the mouse brain B. Vida1, C. S. Molnár1, E. Hrabovszky1, Z. Bardóczi1, A. Caraty2, C. W.3, Z. Liposits1 and I. Kalló1, 4* 1 Institute of Experimental Medicine, Hungary 2 INRA//CNRS/Univ Tours/Haras Nationaux, France 3 King’s College London, School of Medicine, United Kingdom 4 Pázmány Péter Catholic University, Department of Neuroscience, Hungary Kisspeptin (KP) and galanin are important regulators of the GnRH secretion. The current study investigated whether galanin is expressed in KP cells and the galanin peptide is present in KP afferents onto GnRH neurons. Animal models with the highest KP incidence were selected for studying galanin expression in the rostral periventricular area of the third ventricle (RP3V) and the arcuate nucleus (Arc); these were ovariectomised 17β-estradiol-treated (OVX+E2) and OVX oil-treated (OVX+oil) mice, respectively. In situ hybridization revealed the galanin transcript in 39% of KP neurons of the RP3V; the ratio in the Arc was 43%. Double-labeling immunohistochemistry showed galanin immunoreactivity (IR) in 88% of KP neurons in the RP3V and 14% of KP cells in the Arc of colchicine-treated animals. The percentage of double-labeled neurons was significantly lower (5%; p<0.02) in the Arc, but not the RP3V (92%) of male rats. The confocal microscopic analysis was extended to the level of KP axon terminals and showed galanin-IR in ~ 30% of KP appositions onto GnRH neurons in OVX+E2 mice. The co-localization level fell to ~ 5% (p<0.05) in OVX+oil animals. The presence and estrogen-dependent regulation of galanin within KP axon terminals on GnRH neurons add a new aspect to the role played by galanin in the central regulation of reproduction. Acknowledgements This work was supported by OTKA K69127, T73002, K83710; ETT 122/2009 and FP7/2007-2013, grant agreement n° 245009. Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Vida B, Molnár CS, Hrabovszky E, Bardóczi Z, Caraty A, W. C, Liposits Z and Kalló I (2011). Galanin is expressed in kisspeptin neurons and co-localizes with kisspeptin within afferents to GnRH neurons in the mouse brain. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00069 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. I. Kalló, Institute of Experimental Medicine, Budapest, Hungary, kallo@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers B. Vida C. S Molnár E. Hrabovszky Z. Bardóczi A. Caraty C. W. Z. Liposits I. Kalló Google B. Vida C. S Molnár E. Hrabovszky Z. Bardóczi A. Caraty C. W. Z. Liposits I. Kalló Google Scholar B. Vida C. S Molnár E. Hrabovszky Z. Bardóczi A. Caraty C. W. Z. Liposits I. Kalló PubMed B. Vida C. S Molnár E. Hrabovszky Z. Bardóczi A. Caraty C. W. Z. Liposits I. Kalló Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.