Abstract
The intrapancreatic neuropeptide galanin has been demonstrated to lower plasma insulin levels in vivo . The effects of this peptide on insulin secretion, cytoplasmic free Ca 2+ concentration and membrane potential have now been studied in vitro . Glucose-stimulated insulin secretion was inhibited by galanin under these conditions, indicating a direct effect of the peptide on the β-cells. The neuropeptide reversed both the increase in membrane potential and cytoplasmic free Ca 2+ in response to glucose stimulation. At a non-stimulatory concentration of the sugar, galanin induced a slight hyperpolarization without any effect on cytoplasmic free Ca 2+. Galanin did not affect K +-induced increase in cytoplasmic free Ca 2+, excluding a direct inhibitory effect on the voltage-activated Ca 2+ channels. The results indicate that galanin inhibition of glucose-stimulated insulin release involves hyperpolarization with a subsequent decrease in cytoplasmic free Ca 2+.
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