Galanin-containing-neurons, in the gastrointestinal tract of the lizard Podarcis s. sicula, as components of anally projecting nerve pathway

Abstract

The distribution of galanin immunoreactive (Gal/IR) neurons was investigated in the gastrointestinal (GI) tract of the lizard Podarcis s. sicula. The indirect immunofluorescence method, image analysis and confocal analysis were applied to cryostat sections and whole mount preparations. Gal/IR nerve fibers and cell bodies were found throughout the lizard GI tract in the myenteric plexus, circular muscle layer and mucosa. These nerve structures decreased caudally. The stomach revealed a denser reactive nerve population than elsewhere. The projections of Gal/IR neurons were detected in the myenteric plexus of lizard gut using a confocal microscope which analyzed the immunoreactive material on the proximal and distal sides of muscle myotomies. An accumulation of Gal/IR material on the oral side of the myotomies demonstrated the oral-to-anal projection of Gal containing nerve structures. Based on our results, it can be hypothesized that Gal/IR neurons of the lizard digestive tract belong to the inhibitory descending pathway, which in most vertebrates is responsible for gut peristalsis regulation.